| OASL

59 kDa 2'-5'-oligoadenylate synthetase-like protein is an enzyme that is encoded by the OASL gene.2'-5'-oligoadenylate synthase is a protein family of structurally similar proteins, including OAS1, OAS2, and OAS3. However, mutations in the OAS domain mean it lacks the motif to allow oligomerization, preventing the synthesis of oligoadenylates. OASL, like the proteins of 2'-5'-oligoadenylate synthase family, is induced by interferons.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (pg/mL)	1000	500	250	125	62.5	31.2	15.6	0
OD	2.28	1.22	0.74	0.52	0.31	0.24	0.19	0.08
Corrected OD	2.2	1.14	0.66	0.44	0.23	0.16	0.11	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Human OASL spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	81-101	96
Plasma(n=8)	92-105	102
Cell Culture Supernates(n=8)	96-108	104

 

| Linearity

Samples were spiked with high concentrations of Human OASL and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	84-95	85-96	90-110
	Average (%)	91	93	96
1 : 4	Range (%)	89-103	87-108	105-115
	Average (%)	93	98	108